Dear All, I need your guidance in the following issues, Issue No:1I am going to identify two markers on a single cell (macrophage) as well as on two different cells (CD4 and CD8) in a single tissue (costaining). What could be the best secondary conjugate systems for detection in this case (I only have some experience with HRP conjugate). A protocol will be appreciated. Issue No.2what are the specific markers of Kupffer cells? I am working with CD11b and F4/80 for said cells but it seems (found in articles) that these two markers are also expressed by other leukocytes as well, but my job require a very specific marker for kupffer cells so if any one knows please most welcome. Thanks in anticipation.
Nasir Abbas ---PhD FellowGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences190 Kai Yuan Avenue Science Park Guangzhou, 510530, Guangdong P.R.ChinaCell: +86-1312 8296 614 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
