Traditionally we regard nuclear bubbling as incomplete fixation however I'm not so sure that nuclear bubbling can't be caused by additional processing problems. This morning I have some GI biopsies that fixed for nearly 18 hrs that have a large amount of nuclear bubbling. We run the biopsies on a "short run". I am wondering if possibly we are not getting rid of all of the water and therefore our dehydration steps are not long enough?
Anybody have an idea besides incomplete fixation? Jim Jim Vickroy Histology Manager Springfield Clinic, Main Campus, East Building 1025 South 6th Street Springfield, Illinois 62703 Office: 217-528-7541, Ext. 15121 Email: jvick...@springfieldclinic.com<mailto:jvick...@springfieldclinic.com> This electronic message contains information from Springfield Clinic, LLP that may be confidential, privileged, and/or sensitive. This information is intended for the use of the individual(s) or entity(ies) named above. If you are not the intended recipient, be aware that disclosure, copying, distribution, or action taken on the contents of this information is strictly prohibited. If you have received this electronic message in error, please notify the sender immediately, by electronic mail, so that arrangements may be made for the retrieval of this electronic message. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet