We have one girl that always spits on our slide! :) works great! HEATHER SEELEY, HT(ASCP) Histotech 803-985-4676 OFFICE 803-327-7598 FAX
________________________________________ From: Bob Richmond [rsrichm...@gmail.com] Sent: Thursday, May 05, 2016 2:10 PM To: Histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain Amylase (diastase) for the PAS stain queries: Whatever happened to spitting on the slide (30 min at room temperature)? John Kiernan advises "thinking of lemons and drooling into a small beaker" though I'd advise chewing on a rubber band for a few seconds. He notes that alpha amylase is preferred. I'd go with the cheapest one in the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma offers a heat-stable alpha amylase. Bob Richmond Samurai Pathologist Maryville TN _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
