You clearly don't know your histo history. The reason we know that H pylori exists is because a Scientist, Dr. Barry Marshall wanted to prove bacteria caused ulcers and not stress. No one believed him. So he took the organisms from a patient, mixed it in a broth and drank it. He then biopsied himself and treated it. There's a non-uniform method that saved a lot of suffering. Bravo we crazy scientists. Anne
-----Original Message----- From: Geoff via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 12:31 PM To: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] PAS Stain I cannot believe any scientist would advocate such a non-uniform method as spitting on a slide. Buy a bottle of what ever enzyme and use a reproducible buffer and temperature. Geoff On 5/5/2016 3:19 PM, Anne Murvosh via Histonet wrote: > Yes, spitting is the tried and true way to do it. Not to mention no > measuring and cheaper. The reason we switched to a powder is because I just > don't spit well I used to have someone do it for me cause I would end up > drooling. YUCK! The best way to find out is do the amylase method and the > spit method at the same time and have the doctor pick the best. A fun > experiment Anne > > -----Original Message----- > From: Bob Richmond via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Thursday, May 05, 2016 11:36 AM > To: koelli...@comcast.net > Cc: Histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] PAS Stain > > Spokane Ray points out something I've wondered about for years - can just > anybody spit on the slide and remove the glycogen? I've never heard of any > variation, but the number of people I've asked is very limited. This > reference: > http://www.ncbi.nlm.nih.gov/gene/276 > certainly suggests that different people have different salivary alpha > amylase activity. > > Bob Richmond > > On Thu, May 5, 2016 at 2:27 PM, <koelli...@comcast.net> wrote: > >> I love having the Samuri Pathologist on this forum for wisdom and >> real-laboratory life knowledge. And yes, I have in the past spit on slide >> ON OCCASSION when faced with a dire necessity. Although I know there are >> those who would wretch about this; it remains a fact of viable laboratory >> life for some. >> >> My problem now is that in this era of (MUCH TOO MUCH) regulation, how do >> you "test lots" or control from "lot-to-lot variation" in this SOP? When >> Jane or Joe do this routinely and then goes on vacation, what about Sally >> or Jim spit? There is a variation in copy number of the AMY1 gene >> (amylase) and resulting difference in amylase protein concentration amongst >> individuals. >> >> Why not just standardize it from the start, reagent, pH, temperature and >> it really cannot fail. >> >> Spokane Ray >> >> ------------------------------ >> *From: *"Bob Richmond via Histonet" <histonet@lists.utsouthwestern.edu> >> *To: *"Histonet@lists.utsouthwestern.edu" < >> histonet@lists.utsouthwestern.edu> >> *Sent: *Thursday, May 5, 2016 11:10:40 AM >> *Subject: *Re: [Histonet] PAS Stain >> >> >> Amylase (diastase) for the PAS stain queries: >> >> Whatever happened to spitting on the slide (30 min at room temperature)? >> John Kiernan advises "thinking of lemons and drooling into a small beaker" >> though I'd advise chewing on a rubber band for a few seconds. >> >> He notes that alpha amylase is preferred. I'd go with the cheapest one in >> the Sigma-Aldrich catalog. Room temperature is usual, but I note that Sigma >> offers a heat-stable alpha amylase. >> >> Bob Richmond >> Samurai Pathologist >> Maryville TN >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- -- ********************************************** Geoff McAuliffe, Ph.D. Neuroscience and Cell Biology Robert Wood Johnson Medical School 675 Hoes Lane, Piscataway, NJ 08854 voice: (732) 235-4583; fax: -4029 mcaul...@rwjms.rutgers.edu ********************************************** _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
