The KMnO4/oxalic acid sequence will weaken the tissue adherence. If you do not have to use Peroxidase/DAB, then I would recommend alkaline phosphatase labelling (+red chromagen).
Remember KMnO4/oxalic acid can be deleterious to some antigen-antibody reactions. We found that LCA, CAM5.2, L26, alpha-fetoprotein, and NSE were labile, whilst alpha-1-antitrypsin, HBsAg, CMV, CEA, Thyroglobin and chromogranin showed decreased staining. EMA, desmin, HSVII, S100, GFAP, PSA, PAcP, Calcitonin, ACTH and Prolactin were found to be resistant to permanganate treatment. Regards Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) Principal Scientist, the Children's Hospital at Westmead Adjunct Fellow, School of Medicine, University of Western Sydney Tel: 612 9845 3306 Fax: 612 9845 3318 Pathology Department the children's hospital at westmead Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001, Westmead NSW 2145, AUSTRALIA -----Original Message----- From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, 11 May 2016 1:45 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Melanin Bleach Good Morning, I need some help. Yesterday I bleached some heavily pigmented tissue. I have to run some IHC's on them. I bought the bleaching kit from American Master Tech. I had to put the Permanganate for about 6 hours to get the melanin and then a couple of minutes in Oxalic Acid. I had to let them set overnight because I could not get another IHC run in that day. It looks like the tissue fell off during decloaking. I used Apex slides. I rarely ever have to bleach anything here. So I am not sure if I did this correctly. I am thinking I need to bleach and do the run in the same day and not let them set over night in DiH20. Thanks, Karen Heckford HT ASCP CE Lead Histology Technician St. Mary's Medical Center 450 Stanyan St. San Francisco, Ca. 94117 415-668-1000 ext. 6167 karen.heckf...@dignityhealth.org Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you." _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender. Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
