Hi, the blades are packed with a tiny, bit of oil. This can cause you to not obtain a ribbon. Try gently cleaning with xylene, followed by alcohol before sectioning. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953FAX 405-759-7513www.excaliburpathology.com
From: Gareth Davis via Histonet <histonet@lists.utsouthwestern.edu> To: "Histonet@lists.utsouthwestern.edu" <histonet@lists.utsouthwestern.edu> Sent: Tuesday, April 11, 2017 11:55 AM Subject: [Histonet] No Ribbon I feel like I should be able to figure this one out, but I have been having trouble with getting a ribbon for a while now. I have a Leica Microtome RM2125 and I use either a AccuEdge blade (I get one block to cut then it starts to compress) or a StatCut blade (sections do not stick together at all), both High Profile. The angle is about 4 microns. I change the angle a lot to see if that makes a difference and it doesn't. There are so many factors that could be the issue, I think lack of humidity may be the main one. So, suggestions please!! -- Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology Yuma, AZ 85364 928-248-5259 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet