Point taken, thanks Elizabeth. My clinical days are long gone, but that is an important consideration.
yours, mills On Tue, Apr 11, 2017 at 11:13 AM, Elizabeth Chlipala <l...@premierlab.com> wrote: > I might get some individuals upset but we really need to watch what we do > in the histology lab and how that effects the precision of our process. > When you breath on the block you are basically generating a thicker > section, it's not a good practice to keep. Trust me I used to do this in > the past. Thicker sections especially for IHC will generate a more intense > stain, most individuals may think that that should not be an issue but it > is - in the clinical setting especially when you are staining for > therapeutic markers such as Her2 and PD-L1 - 510K cleared kits for these > recommend specific section thickness settings and in the research setting > if you are running any type of analysis on a batch of samples. If your > microtome is set at 4 microns and you are breathing on the block you are > not cutting at 4 microns, you are cutting thicker than that and a thicker > section will increase staining intensity and therefore alter the results. > > Just my two cents. > > Liz > > Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC > Premier Laboratory, LLC > PO Box 18592 > Boulder, CO 80308 > (303) 682-3949 office > (303) 682-9060 fax > (303) 881-0763 cell > l...@premierlab.com > www.premierlab.com > > Ship to Address: > > Premier Laboratory, LLC > 1567 Skyway Drive, Unit E > Longmont, CO 80504 > > > -----Original Message----- > From: Caroline Miller via Histonet [mailto:histonet@lists. > utsouthwestern.edu] > Sent: Tuesday, April 11, 2017 11:59 AM > To: Paula Keene Pierce > Cc: Histonet Histonet > Subject: Re: [Histonet] No Ribbon > > Yes, +1 to Paula, also: > - I often found they are too sharp (!!) to ribbon so a gentle wipe (in the > direction of the bevel) with a kimwipe helps > - Also 'huffing' slightly on the block will warm the paraffin and both > smooth out the wrinkles as well as make the sections stick together > > :) > > mills > > On Tue, Apr 11, 2017 at 10:37 AM, Paula Keene Pierce via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > > > Hi, > > the blades are packed with a tiny, bit of oil. This can cause you to > > not obtain a ribbon. > > Try gently cleaning with xylene, followed by alcohol before > > sectioning. Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur > > Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH > > 405-759-3953FAX 405-759-7513www.excaliburpathology.com > > > > From: Gareth Davis via Histonet > > <histonet@lists.utsouthwestern.edu> > > To: "Histonet@lists.utsouthwestern.edu" <histonet@lists. > > utsouthwestern.edu> > > Sent: Tuesday, April 11, 2017 11:55 AM > > Subject: [Histonet] No Ribbon > > > > I feel like I should be able to figure this one out, but I have been > > having trouble with getting a ribbon for a while now. I have a Leica > > Microtome > > RM2125 and I use either a AccuEdge blade (I get one block to cut then > > it starts to compress) or a StatCut blade (sections do not stick > > together at all), both High Profile. The angle is about 4 microns. I > > change the angle a lot to see if that makes a difference and it > > doesn't. There are so many factors that could be the issue, I think > > lack of humidity may be the main one. So, suggestions please!! > > > > -- > > Ms. Gareth B. Davis, B.S., HT, QIHC (ASCP)cm Yuma Gastroenterology > > Yuma, AZ 85364 > > 928-248-5259 > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > > > _______________________________________________ > > Histonet mailing list > > Histonet@lists.utsouthwestern.edu > > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > > > > -- > Caroline Miller (mills) > Director of Histology > 3Scan.com > 415 2187297 > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > ______________________________________________________________________ > This email has been scanned by the Symantec Email Security.cloud service. > For more information please visit http://www.symanteccloud.com > ______________________________________________________________________ > -- Caroline Miller (mills) Director of Histology 3Scan.com 415 2187297 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet