> > Hi Reuel > We had the same thing as you. It was caused by bad MMA, I tested. Write down the lot # and ask refund. Test new lot # 1st of MMA in the future. It was very frustrating....I know. Best luck.
Dorothy > Message: 2 > Date: Mon, 12 Feb 2018 21:07:15 +0000 > From: Reuel Cornelia <reuel.corne...@tsrh.org> > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Deplastifying MMA > Message-ID: > <SN1PR17MB03668392B3AC2DDACA8FBA92E7F70@SN1PR17MB0366. > namprd17.prod.outlook.com> > > Content-Type: text/plain; charset="iso-8859-1" > > Hello histonetters, > > I have a problem with my MMA plastic section on my slide. I could not > remove the plastic MMA even if I leave the section slides in Xylene for > several days with heat incubation at 60 degrees. This is the first time > that happened to my section for several years of doing the same thing over. > I was thinking that it was my Xylene lot number so I tried to change it but > still does not remove the plastic, and then Itried different solvent > Acetone, and Ethylene glycol Monoethyl ether but still it does not work. > Can I ask for help if anyone knows what was going on and what would be the > best way to remove this plastic from my section? > > Just to give you my embedding solution was MMA -94% , dibutly phthalate- > 5% and perkadox 16- 0.5%. This have been my solution for years and I do not > have any problem with the removal of plastic section until now. I was > thinking that my MMA (M55909) different lot number from Sigma Aldrich may > have cause this because even I tried to use the MMA to dissolve my plastic > it does not work. > > > Thank you for and any opinions or protocols are greatly appreciated. > > > Reuel Cornelia > > TSRH > > 214-559-7766 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet