Hi Dorothy, Hi Reuel, MMA embedding and deplastination is of course a very difficult process and many protocols are discussed, but only a few work well. So in general: real deplastination cannot be performed by xylene or acetone alone, these solutions are just a pretreatment. The best way for remove MMA from section is a procedure starting with xylene, then going into acetone (2 times) and then into MEA (Methoxyethylacetate) for at least 2-3 times for a minimum of 10-20 minutes. You can also directly go into MEA, however, pretreatment with xylene or acetone helps to reduce the steps and volume of MEA you need.
Which kind of MMA do you use? In our lab we do resin embedding in Technovit 9100, which is very well established MMA that provides good results for thin sections between around a few microns, but and also for thicker sections and even for cutting-grinding-techniques. We can also provide you with several protocols for infiltration, deplastination and staining of Technovit-sections. You find some further information and also some hands-on-descriptions for all histology-technovits in this brochure: http://www.morphisto.de/fileadmin/pdf/Brochure_Technovit_Histology_EN.pdf <http://www.morphisto.de/fileadmin/pdf/Brochure_Technovit_Histology_EN.pdf> If you have any further questions, just contact me personally. With best regards Michael P.S. Further information about the technovits you can also find here: http://www.morphisto.de/en/resin-histology/technovit-resins/ <http://www.morphisto.de/en/resin-histology/technovit-resins/> > Am 14.02.2018 um 20:45 schrieb Dorothy Hu via Histonet > <histonet@lists.utsouthwestern.edu > <mailto:histonet@lists.utsouthwestern.edu>>: > >> >> Hi Reuel >> > > We had the same thing as you. It was caused by bad MMA, I tested. Write > down the lot # and ask refund. Test new lot # 1st of MMA in the future. It > was very frustrating....I know. > Best luck. > > Dorothy > > > > > >> Message: 2 >> Date: Mon, 12 Feb 2018 21:07:15 +0000 >> From: Reuel Cornelia <reuel.corne...@tsrh.org >> <mailto:reuel.corne...@tsrh.org>> >> To: "histonet@lists.utsouthwestern.edu >> <mailto:histonet@lists.utsouthwestern.edu>" >> <histonet@lists.utsouthwestern.edu >> <mailto:histonet@lists.utsouthwestern.edu>> >> Subject: [Histonet] Deplastifying MMA >> Message-ID: >> <SN1PR17MB03668392B3AC2DDACA8FBA92E7F70@SN1PR17MB0366. >> namprd17.prod.outlook.com <http://namprd17.prod.outlook.com/>> >> >> Content-Type: text/plain; charset="iso-8859-1" >> >> Hello histonetters, >> >> I have a problem with my MMA plastic section on my slide. I could not >> remove the plastic MMA even if I leave the section slides in Xylene for >> several days with heat incubation at 60 degrees. This is the first time >> that happened to my section for several years of doing the same thing over. >> I was thinking that it was my Xylene lot number so I tried to change it but >> still does not remove the plastic, and then Itried different solvent >> Acetone, and Ethylene glycol Monoethyl ether but still it does not work. >> Can I ask for help if anyone knows what was going on and what would be the >> best way to remove this plastic from my section? >> >> Just to give you my embedding solution was MMA -94% , dibutly phthalate- >> 5% and perkadox 16- 0.5%. This have been my solution for years and I do not >> have any problem with the removal of plastic section until now. I was >> thinking that my MMA (M55909) different lot number from Sigma Aldrich may >> have cause this because even I tried to use the MMA to dissolve my plastic >> it does not work. >> >> >> Thank you for and any opinions or protocols are greatly appreciated. >> >> >> Reuel Cornelia >> >> TSRH >> >> 214-559-7766 >> >> > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu <mailto:Histonet@lists.utsouthwestern.edu> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet ************************************************************************************************ MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weismüllerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.g...@morphisto.de <mailto:i...@morphisto.de> Internet: http://www.morphisto.de/ <http://www.morphisto.de/> Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. ************************************************************************************************ MORPHISTO GmbH PD Dr. phil. nat. Michael Gudo Weismüllerstr. 45 60314 Frankfurt am Main Telefon: 069 / 400 3019 - 62 Telefax: 069 / 400 3019 - 64 E-Mail: michael.g...@morphisto.de <mailto:i...@morphisto.de> Internet: http://www.morphisto.de/ <http://www.morphisto.de/> Vertretungsberechtigter Geschäftsführer: Dr. Michael Gudo Registergericht: Amtsgericht Frankfurt Registernummer: HRB 74954 Umsatzsteuer-Identifikationsnummer gemäß § 27 a Umsatzsteuergesetz: DE243397199 ************************************************************************************************ Diese Nachricht ist ausschliesslich fuer den bezeichneten Adressaten oder dessen Vertreter bestimmt. Beachten Sie bitte, dass jede Form der unautorisierten Nutzung, Veroeffentlichung, Vervielfaeltigung oder Weitergabe des Inhaltes der Email nicht gestattet ist. Sollten Sie nicht der vorgesehene Adressat dieser Email oder dessen Vertreter sein, so bitten wir Sie, sich mit dem Absender der Email in Verbindung zu setzen und anschliessend diese Email und saemtliche Anhaenge zu loeschen. ************************************************************************************************ This message is exclusively for the person addressed or their representative. Any form of the unauthorized use, publication, reproduction, copying or disclosure of the content of this e-mail is not permitted. If you are not the intended recipient of this message and its contents, please notify this sender immediately and delete this message and all its attachments subsequently. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
