Hi Reuel, One thing you could try is THF (tetrahydrofuran). We have used it in the past to rapidly dissolve the MMA on slides.
Good luck, Lori -----Original Message----- From: Dorothy Hu via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Wednesday, February 14, 2018 11:46 AM To: histonet@lists.utsouthwestern.edu Subject: [EXTERNAL] [Histonet] Deplastifying MMA > > Hi Reuel > We had the same thing as you. It was caused by bad MMA, I tested. Write down the lot # and ask refund. Test new lot # 1st of MMA in the future. It was very frustrating....I know. Best luck. Dorothy > Message: 2 > Date: Mon, 12 Feb 2018 21:07:15 +0000 > From: Reuel Cornelia <reuel.corne...@tsrh.org> > To: "histonet@lists.utsouthwestern.edu" > <histonet@lists.utsouthwestern.edu> > Subject: [Histonet] Deplastifying MMA > Message-ID: > <SN1PR17MB03668392B3AC2DDACA8FBA92E7F70@SN1PR17MB0366. > namprd17.prod.outlook.com> > > Content-Type: text/plain; charset="iso-8859-1" > > Hello histonetters, > > I have a problem with my MMA plastic section on my slide. I could not > remove the plastic MMA even if I leave the section slides in Xylene > for several days with heat incubation at 60 degrees. This is the first > time that happened to my section for several years of doing the same thing > over. > I was thinking that it was my Xylene lot number so I tried to change > it but still does not remove the plastic, and then Itried different > solvent Acetone, and Ethylene glycol Monoethyl ether but still it does not > work. > Can I ask for help if anyone knows what was going on and what would be > the best way to remove this plastic from my section? > > Just to give you my embedding solution was MMA -94% , dibutly > phthalate- 5% and perkadox 16- 0.5%. This have been my solution for > years and I do not have any problem with the removal of plastic > section until now. I was thinking that my MMA (M55909) different lot > number from Sigma Aldrich may have cause this because even I tried to > use the MMA to dissolve my plastic it does not work. > > > Thank you for and any opinions or protocols are greatly appreciated. > > > Reuel Cornelia > > TSRH > > 214-559-7766 > > _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet [CONFIDENTIALITY AND PRIVACY NOTICE] Information transmitted by this email is proprietary to Medtronic and is intended for use only by the individual or entity to which it is addressed, and may contain information that is private, privileged, confidential or exempt from disclosure under applicable law. If you are not the intended recipient or it appears that this mail has been forwarded to you without proper authority, you are notified that any use or dissemination of this information in any manner is strictly prohibited. In such cases, please delete this mail from your records. To view this notice in other languages you can either select the following link or manually copy and paste the link into the address bar of a web browser: http://emaildisclaimer.medtronic.com _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
