Some facilities run their immunostainers on a continuous basis all week long and antibodies may never leave the machine for multiple days. The antibody is still valid and still works. Like a previous responder said you can test each one and compare to previously stained slides to validate it is still working. I don't remember where I heard it but someone published that for each hour an antibody is not keep within it's storage temperature range you lose 1 day of effective usage. (so if the antibodies were out of temp for 24 hours you lose 24 days from the printed expiration date)
On Wed, Mar 27, 2019 at 1:24 PM MONICA D. LOCKHART via Histonet < histonet@lists.utsouthwestern.edu> wrote: > I agree !! The integrity of the antibody is suspect after being exposed to > temps like that. Even if the fridge door was kept closed during the > outtage. > > Monica D. Lockhart > > > -----Original Message----- > From: WILLIAM DESALVO [mailto:wdesalvo....@outlook.com] > Sent: Tuesday, March 26, 2019 7:20 PM > To: MARY ANN; histonet@lists.utsouthwestern.edu > Subject: Re: [Histonet] Fridge temp > > Simple, revalidate or pitch > > William DeSalvo > ________________________________ > From: MARY ANN via Histonet <histonet@lists.utsouthwestern.edu> > Sent: Tuesday, March 26, 2019 4:09:01 PM > To: histonet@lists.utsouthwestern.edu > Subject: [Histonet] Fridge temp > > Let's say, hypotheticaly, if you discover your fridge with all you > antibodies and detection kits were discovered to have been at 19c. for an > undisclosed amout of time. 12 24 48 hours due tona power surge..south > Florida weather. > > Let's also propose your lab CFO/Owner dosent think its a big deal. > > First how would you handle the issue given the frisge has been restored ? > > > > > Sent from Xfinity Connect Application > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > > > Confidentiality Notice: > This e-mail, including any attachments is the property of Trinity Health > and is intended for the sole use of the intended recipient(s). It may > contain information that is privileged and confidential. Any unauthorized > review, use, disclosure, or distribution is prohibited. If you are not the > intended recipient, please delete this message, and reply to the sender > regarding the error in a separate email. > > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet > -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet