Terri is right on the money with her advice. Especially the de- ionized water, NO double dipping and defiantly NO adhesive part.
John Sent from ProtonMail Mobile On Wed, Jul 17, 2019 at 10:25 AM, Terri Braud via Histonet <histonet@lists.utsouthwestern.edu> wrote: > Hi Deanne - > Hope this will help > For our Ventana Ultra, we use Leica / Surgipath Apex Superior Adhesive slides. > We use de ionized water in our baths at 45C ish, no additives. > We never "double-dip" our slides (meaning that if we mount a control on the > top of the slide, we turn the slide upside down to pick it up, only immersing > the slide to pick up the intended tissue) > We pre-bake IHC slides in a hot air blower over at 65C, 10 min for small > tissues, and 1 hr for large tissues, then load for IHC. > > For special stains, we use the same slides, but they go directly on the > Sakura Prisma stainer into the instrument oven at 65C for 15 minutes before > staining > For H&E, we use the plain Surgipath Snowcoat slides, no adhesive, directly on > the Sakura Prisma stainer into the instrument oven at 65C for 15 minutes > before staining. They dry vertically in a rack as we cut, then we load as > soon as the rack is full. > > We once had an issue with tissue falling off and were able to trace it to a > new tech's liberal use of Paraguard Spray. Stopped using it, wiped everything > down with alcohol and our problem went away. > Also, many years ago, I had a problem with a bad lot of slides, but these > were Cardinal's. > I sincerely hope you can get this straightened out. I know how frustrating > such a problem can be. > Terri > > Terri L. Braud, HT(ASCP) > Anatomic Pathology Supervisor > Laboratory > Holy Redeemer Hospital > 1648 Huntingdon Pike > Meadowbrook, PA 19046 > ph: 215-938-3689 > fax: 215-938-3874 > Care, Comfort, and Heal > > Today's Topics: > 2. Tissue Adherence Issue (Knutson, Deanne) > Message: 2 > Date: Wed, 17 Jul 2019 09:21:49 -0500 > From: "Knutson, Deanne" <dknut...@primecare.org> > Subject: [Histonet] Tissue Adherence Issue > Fellow Histonetters - > > I would appreciate your feedback on an intermittent issue that has shown up > in our lab. > All of a sudden, we are having difficulty with tissue specimens falling off > of our slides on the IHC stains and special stains sporadically. > About a year ago, we switched instruments on the IHC bench from the Leica > BOND to the ROCHE ULTRA, and we use the Ventana NexES special stainer. > No adherence issues with all of the validation slides that were run. > We have tried various types of slides recently as well, and the issue still > prevails. > > Would any of you mind telling me your slide workflow - type of slide used, > gelatin or not used in flotation bath, how long slides are cooked, etc.... > for your IHC slides, for your special stains slides, and even for your H&E > slides. > I would welcome your suggestions and feedback. > > Thank you so much!!! > Deanne Knutson > Supervisor > Anatomic Pathology > "Let All Be Received as Christ." > > _______________________________________________ > Histonet mailing list > Histonet@lists.utsouthwestern.edu > http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
