Good afternoon Histoneters, I have been doing IHC and IF during decades in rodent brain tissue, always in free floating. Now, I would like to do that sections placed over slides and perform the whole procedure over the slide. I am interested in using thick sections, usually I used 50micrometers, what is the maximum thickness that I could use in slide to guarantee the correct penetration of the antibodies and the ABC complex. Before the IHC I always use graded alcohols and xilene, and antigen retrieval with citrate buffer. Thank you!
-- Dr. Alonso Martínez Canabal PhD Profesor Asociado "C" Departamento de Biología Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet