Hi, thank you for your reply. I cannot do my usual free floating staining because this tissue is very brittle. So, I was thinking to do the staining in slide at 30 microns? Thank you
El vie, 4 ago 2023 a las 12:29, Colleen Forster (<cfors...@umn.edu>) escribió: > Alonso, > > For sections that thick you would collect them as you always have, stain > them as floating sections and then mount onto a glass slide. The routine > standard methods for IHC will never penetrate a 50um thick section. > > Colleen Forster HT(ASCP)QIHC > > > > On Fri, Aug 4, 2023 at 1:17 PM Alonso Martínez Canabal via Histonet < > histonet@lists.utsouthwestern.edu> wrote: > >> Good afternoon Histoneters, >> I have been doing IHC and IF during decades in rodent brain tissue, >> always in free floating. Now, I would like to do that sections placed over >> slides and perform the whole procedure over the slide. I am interested in >> using thick sections, usually I used 50micrometers, what is the maximum >> thickness that I could use in slide to guarantee the correct penetration >> of >> the antibodies and the ABC complex. Before the IHC I always use graded >> alcohols and xilene, and antigen retrieval with citrate buffer. >> Thank you! >> >> -- >> Dr. Alonso Martínez Canabal PhD >> Profesor Asociado "C" >> Departamento de Biología Celular, Facultad de Ciencias, UNAM >> Investigador Nacional "I" >> 56224833 >> _______________________________________________ >> Histonet mailing list >> Histonet@lists.utsouthwestern.edu >> http://lists.utsouthwestern.edu/mailman/listinfo/histonet >> > > > -- > Colleen Forster HT(ASCP)QIHC > BLS Histology and IHC Laboratory > Jackson Hall, Room 2-155 > 321 Church St. SE > Minneapolis, MN 55455 > 612-626-1930 > > > > > > > -- Dr. Alonso Martínez Canabal PhD Profesor Asociado "C" Departamento de Biología Celular, Facultad de Ciencias, UNAM Investigador Nacional "I" 56224833 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet