Just a point of clarification .. what Mungo said isn't quite right:
On 14/12/2015 11:24, Mungo Carstairs (Staff) wrote:
> By default this will use the first sequence as the reference sequence.

The 'Show nonconserved' option highlights differences to the currently
defined reference sequence, or the consensus for each column when no
reference is defined.

Hope that helps!
Jim.
ps. You can define a reference sequence for each group on the alignment,
useful when working with multiple sets of closely related sequences.
-- 
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Dr JB Procter, Jalview Coordinator, The Barton Group
Division of Computational Biology, School of Life Sciences
University of Dundee, Dundee DD1 5EH, UK.
+44 1382 388734 | www.jalview.org | www.compbio.dundee.ac.uk

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