Greetings netters I am trying to do some surface display on PhiX174 so I started with what should have been a simple control. I bought PhiX174 replicative form 1 from Frmentas, got some E. coli C (HF4704) from the E. coli stock center at Yale (Thanks again guys!), mixed phage DNA + mid log HF4704 + top agar and poured on LB plates. Left at 37C O.N. and got nice growth but no Plaques. Any idea what am I doing wrong?
Many thanks for any advice Yoram ------------------------------------------------------------------------ This message was sent using IMP, the Webmail Program of Haifa University _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
