Hi, I have a question about isolating circular ssDNA from plasmids carrying the f1 phage replication origin - does anyone know if there is a size limit for the insert that can be subcloned into the MLS? The problem is I'm working with very big mammalian genomic DNA inserts (usually 13-16 kb)...and need to grow them in a plasmid with the p15A ori. Also, does anyone know how to linearize the circular ssDNA obtained from M13 helper phage-infected bacteria?
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