Maybe Nile Blue, or Neutral Red<http://www.histosearch.com/histonet/Jul00A/Re.fastredneutralornuclea.html> .
Sébastien Vigneau On Mon, Dec 28, 2009 at 1:40 PM, merav karsenty <[email protected]> wrote: > I need the stain to be non fluorecent because I dont have acess to a > fluorecent microscop. I know Haematoxylin, but as I anderstand, using this > stain must involve fixation. I am working with living cells and I am > trying > to find a way to stain them without fixation, unless there is no choice. Is > there a way that you know? > > 2009/12/28 Nick Theodorakis <[email protected]> > > > On Dec 27, 9:51 am, Sébastien Vigneau <[email protected]> > > wrote: > > > Haematoxylin? > > > > I was thinking of that, too. > > > > Some clarification from the OP would help, too. Is it that you don't > > have access to a fluorescent scope, or that you don't want the stain > > to be fluorescent? Because if it is the latter, I believe that > > hematoxylin is autofluorescent; or at least, some component of an H&E > > stained slide is autofluorescent. > > > > What is the nature of your sample? Live, fixed or frozen? Cells or > > tissues? How are you visualizing it now? > > > > Nick > > > > -- > > Nick Theodorakis > > [email protected] > > contact form: > > http://theodorakis.net/contact.html > > _______________________________________________ > > Methods mailing list > > [email protected] > > http://www.bio.net/biomail/listinfo/methods > > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
