On Tue, 2 Feb 2010 09:51:09 -0800 (PST), WS <[email protected]> wrote:
>Many thanks for your suggestions so far! > >I forgot to mention that (except to my normal behavior in the methods >+reagents realm where I like to suggest homebrew), this time I am in >need of a solution that does not require any calibration etc. etc. I >need reproducibility. I wondered if there was any commercial >alternative to PD-10, preferably even of the same dimensions (although >this wouldn't be a problem as long as they are similar scale and have >a frit on top). I searched the web, of course, but haven't found any >alternative. . > >Wo You could also try Ammonium sulfate precipitation of your protein and then resuspend the pellet in your buffer of choice. _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
