Am 15.02.2010, 10:53 Uhr, schrieb Yvonne Couch
<[email protected]>:
Hi all,
I'm trying to get a peptide into solution, it currently only goes into
solvent and high percentage DMSO but not into saline. I want to run a
small
volume dialysis since its final volume will be only around 200ul. Will
this
get rid of the DMSO or will it come out of solution? Does anyone have
any
experience with small volume dialysis and can you give me some tips?
Yes, the DMSO will come out and yes, the peptide will probably precipitate
again. With that sort of volume, you probably want to use commercial
dialysing equipment, e.g. Slide-A-lyser from Pierce or the like. You could
also try to float the membrane on the surface of the buffer, and spot your
solution onto it in a small drop. However, recovery requires a steady hand
and some practice.
What I would do instead, however, is to have the peptide in a high
concentration stock solution in DMSO and dilute that into my assay so that
the final DMSO concentration is, say, 1%. That does not affect most
bio-assays, and obviously, I would include 1% DMSO in my controlls too.
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