Am 03.03.2010, 16:24 Uhr, schrieb Pow Joshi <[email protected]>:
Hi Everyone,
I was just wondering if anyone would have some "Insider's" knowledge
about
the fixative "Histochoice". It is supposed to be a swanky non-formalin
based
fixative for all and sundry, and the ingredients are supposed to be
"undisclosed" :). I am guessing it is some kind of
methanol/acetone/ethanol/aldehyde based solution.
Either way, I would also appreciate insights about its efficiency for
Lipid
based dyes such as Sudan red class of dyes or DiI/DiO if anyone has tried
using it in combination with this fixative.
If the composition is undisclosed, do not use it. Science requires
reproducibility, and what happens if the manufacturer goes out of busyness
or replaces "Histochoice" with "Histochoice Plus"?
The following reference contains everything about microscopy-reagents you
will ever want to know:
@book{Rom-89,
AUTHOR= {B. Romeis},
TITLE= {Mikroskopische {T}echnik},
YEAR= {1989},
PUBLISHER= {Urban und Schwarzenberg},
ADDRESS= {München},
EDITION= {17}
}
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