Thank you DK, Engelbert... It's strange when I looked at the MSDS I did'nt find any details; on hindsight I'm guessing I probably looked at what Sigma has on it's website not Amresco directly. Probably, it is best to do the staining and check what the results are :)
Pow On 10 March 2010 23:24, DK <[email protected]> wrote: > In article <[email protected]>, Pow Joshi < > [email protected]> wrote: > >Hi Everyone, > > > >I was just wondering if anyone would have some "Insider's" knowledge about > >the fixative "Histochoice". It is supposed to be a swanky non-formalin > based > >fixative for all and sundry, and the ingredients are supposed to be > >"undisclosed" :). I am guessing it is some kind of > >methanol/acetone/ethanol/aldehyde based solution. > > It's glyoxal-based. Look at the chemical composiion in the MSDS: > http://www.nbsbio.co.uk/downloads/MSDS%20HistoChoice%20MB.pdf > You can figure out [probably] everything you need to know about > it by going to Google patents and reading US patents #5439667 > and 5,429,797. > > >Either way, I would also appreciate insights about its efficiency for > Lipid > >based dyes such as Sudan red class of dyes or DiI/DiO if anyone has tried > >using it in combination with this fixative. > > No idea. My chemically naive guess would be "better than formalin > but milder than glutaraldehyde". > > DK > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
