Hi all, I'm currently trying to perform immunostaining on C2C12 skeletal muscle cells which have been plated onto a 6-well cell culture plate. The antibody that I am using has been used in my lab previously on cryo-sectioned tissue slices which were not fixed before staining. It has been suggested to me that I should not fix the cells before staining.
Is there a reliable way to perform IHC on cell monolayers without fixation? I am not experienced in IHC and but I've had success in using fixatives such as PAF for various staining protocols. Does anyone have a protocol they'd be willing to share that doesn't require fixation? Thanks! J _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
