Read up on this: http://www.ihcworld.com/immunocytochemistry.htm
They discuss how to stain living cells growing in a dish. There are also some fairly gentle fixatives such as methanol or acetone that very often retain antigenicity. Adam On Tue, Mar 9, 2010 at 7:29 PM, Joel Schneider <[email protected]>wrote: > Hi all, > > I'm currently trying to perform immunostaining on C2C12 skeletal muscle > cells which have been plated onto a 6-well cell culture plate. The antibody > that I am using has been used in my lab previously on cryo-sectioned tissue > slices which were not fixed before staining. It has been suggested to me > that I should not fix the cells before staining. > > Is there a reliable way to perform IHC on cell monolayers without fixation? > I am not experienced in IHC and but I've had success in using fixatives > such > as PAF for various staining protocols. Does anyone have a protocol they'd > be > willing to share that doesn't require fixation? > > Thanks! > > J > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
