Dear all,
Some time ago I used an E. coli cell line (supposed to be some kind of Origami
strain) to express a protein.
After having trying different cells, this one was the only where I could
clearly see the band corresponding to my protein.
Unfortunately I didn't write down the strain used and it seems that the
expression disappears if glycerolates are reused.
Some months later I have tried again to express the protein using different
Origami available at my workplace. But I could never reproduce my previous
result.
Therefore it would be really useful if I could identify the cells used the
first time. Can I do some kind of DNA/RNA protein or other analysis to get this
information?
I am becoming desperate since I don't know what else can be done.
Thank you very much,
Eva
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