Hi Lebo, a) have you tried to re-amplify an aliquot of your PCR product? Transfer say 1µl of your reaction into a new one. b) do you get stronger bands with that locus when cloned into a plasmid? -> then check fpor PCR inhibitors in your sample.
HTH Wo _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
