Many years ago, I used to do some methylation validation on genomic DNA using bisulfite treatment followed by restriction digest. The only way we could get it to work was nested PCR. I think that re-amplifying your PCR product is probably your best bet.
Adam On Thu, Apr 22, 2010 at 9:53 AM, WS <[email protected]> wrote: > Hi Lebo, > > a) have you tried to re-amplify an aliquot of your PCR product? > Transfer say 1µl of your reaction into a new one. > b) do you get stronger bands with that locus when cloned into a > plasmid? -> then check fpor PCR inhibitors in your sample. > > HTH > > Wo > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
