Hi, just add as much as you like. I have saturated solution of phenol red (some material not dissolved) in water, shake it well before use and the add 1 or 2 drops. You may also add it to the separation gel, as you then may monitor the front very easily. In contrast to bromophenolblue phenol red polymerizes to some extent into the gel, then it's easier to locate and remove air bubbles in blots.
Have fun! Wo _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
