In article <[email protected]>,
[email protected] says...
>
> Hello!
>
>
>
> I have seen some SDS PAGE gels with red or orange stacking part that makes
> the wells visible. They use phenol red for the red stacking gel. But I
> couldn't find the concentration of phenol red they use for this purpose.
> Does anybody have any experience in this?
See
@ARTICLE{Smi-88,
author = {I. Smith and R. Cromie and K. Stainsby},
title = {Seeing gel wells well},
journal = {Anal. Biochem.},
volume = {169},
year = {1988},
pages = {370-371},
doi = {10.1016/0003-2697(88)90297-7},
language = {engl},
}
Note however, that this works only with negative detergents like SDS. If
you do electrophoresis with positively charged detergents like CTAB, you
need a different dye:
@INPROCEEDINGS{Bux-09,
author = {E. Buxbaum},
title = {Cationic electrophoresis and eastern blotting},
chapter = {14},
pages = {115-128},
editor = {B.T. Kurien and R.H. Scofield},
booktitle = {Protein blotting and detection -- {M}ethods and
protocols},
publisher = {Humana},
year = {2009},
volume = {536},
series = {Meth. Mol. Biol.},
isbn = {978-1-934115-73-2},
address = {Dordrecht},
language = {engl},
}
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