You could try Rytas Vilgalys' website. It has all of the rDNA primers, subdivided into groups 28S, ITS, 18S. For 28S, it says that most molecular systematics studies only utilize the first 600-900 bases from the LSU gene .... We covered part of this using ITS1F and LR3. Vilgalys describes how they amplify this region and what primers they use for sequencing.
This is the web address: http://www.biology.duke.edu/fungi/mycolab/primers.htm Quoting Adroit A <[email protected]>:
Dear Bionetters, I am trying to amplify the 28s rRNA region of a fungus. For this i already isolated the genomic DNA and amplified it with ITS-5 and ITS-4 primers. I got a PCR product of length approx. 700bp. However this amplified product covers the region between 18s-5.8s-28s. Could anyone please suggest me the universal primers that specifically amplify the 28s region alone? I also used ITS-1 and ITS-4 primers for amplification, however no amplification was observed. Please let me know the reason for this. Thanking you in advance. Thanks, Adroit _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
Ale Mandel _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
