On Aug 17, 4:17 am, Adroit A <[email protected]> wrote: > Dear Bionetters, Dear Adroit,
when you re-run the extracted bands on a gel, what do you see? Do you still get the expected size? You might try the freeze-squeeze method instead. Wo > > I am facing some problem with sequencing of PCR products that are gel > extracted. On sequencing i am not getting the complete sequence of the PCR > products (even though the size of the PCR products is small, 200 -1000bp). > There is a slow drop is the sequence and i fail to get complete sequence. > > I am following the Qiagen protocol for gel extraction. > > Please let me know a possible reason for this and also suggest a solution. > > Thanking you in advance. > > Regards, > Adroit _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
