Hello all, I am considering the development of a plasmid-based bacterial transformation/expression vector that would sidestep the use of antibiotic selection in a range of cell types. I was hoping someone on this list could offer some insight into current methods that avoid the use of antibiotics?
I know some methods call for the use of strains lacking the ability to grow on media without X amino acid, and complement this deficiency with a plasmid-encoded X-synthase. While this is very clever, it does restrict work to appropriate strains only, which is too inflexible for me. What else is out there, besides simply diluting and streaking cells hoping for a positive single colony? Thanks, Cathal Garvey -- letters.cunningprojects.com twitter.com/onetruecathal twitter.com/labsfromfabs _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
