Dear all, I'm running cell lysate on superose 6 followed by SDS-PAGE WB. While my protein of interest is detected as a single band in the unfractionated lysate (i.e. total protein), it reproducibly migrates as two discrete bands in superose 6 fractions.
Now, this is not a matter of relative concentration, because the two bands occur in ALL superose 6 fractions and cannot be detected in unfractionated lysate, even at up to 200ug total protein! My lysis buffer contains protease and phosphatase inhibitors, so this shouldn't be the problem. Any ideas as to why gel filtration chould affect the migration pattern of an individual protein? Thanks, Ranen Aviner _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
