About 50 and 55 kDa. Ranen On Thu, Nov 11, 2010 at 7:04 PM, <[email protected]>wrote:
> Send Methods mailing list submissions to > [email protected] > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/methods > or, via email, send a message with subject or body 'help' to > [email protected] > > You can reach the person managing the list at > [email protected] > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Methods digest..." > > > Today's Topics: > > 1. Separation by Superose 6 gel filtration (ranen aviner) > 2. Re: Separation by Superose 6 gel filtration (WS) > > > ---------------------------------------------------------------------- > > Message: 1 > Date: Thu, 11 Nov 2010 11:14:22 +0200 > From: ranen aviner <[email protected]> > Subject: Separation by Superose 6 gel filtration > To: [email protected] > Message-ID: > > <[email protected]<aanlktiny3ky7ay77oh2uzfcza1oelo2up311xennl%[email protected]> > > > Content-Type: text/plain; charset=ISO-8859-1 > > Dear all, > > I'm running cell lysate on superose 6 followed by SDS-PAGE WB. While my > protein of interest is detected as a single band in the unfractionated > lysate (i.e. total protein), it reproducibly migrates as two discrete bands > in superose 6 fractions. > > Now, this is not a matter of relative concentration, because the two bands > occur in ALL superose 6 fractions and cannot be detected in unfractionated > lysate, even at up to 200ug total protein! > > My lysis buffer contains protease and phosphatase inhibitors, so this > shouldn't be the problem. > > Any ideas as to why gel filtration chould affect the migration pattern of > an > individual protein? > > Thanks, > Ranen Aviner > > > ------------------------------ > > Message: 2 > Date: Thu, 11 Nov 2010 07:15:13 -0800 (PST) > From: WS <[email protected]> > Subject: Re: Separation by Superose 6 gel filtration > To: [email protected] > Message-ID: > <[email protected]> > Content-Type: text/plain; charset=ISO-8859-1 > > What about the size of the bands you get? > > Wo > > > ------------------------------ > > _______________________________________________ > Methods mailing list > [email protected] > http://www.bio.net/biomail/listinfo/methods > > End of Methods Digest, Vol 66, Issue 6 > ************************************** > _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
