Hi all,
I am using Waters silica C18 column for my analyses. It recently started
showing a much higher pressure for ACN at 1.0ml/min, around 100 as compared
to normal which is 45. HPLC profiles are not affected though, I am getting
good peaks.
Some colleagues suggested that I give it a wash while it is fixed upside
down at a higher flow rate. When I did that, it showed me a back pressure of
50 at 1.0ml/min. But when I flipped it back to the right orientation, it
still showed me a back pressure of 100 at 1.0ml/min flow rate....
Any ideas what could be going on? I am very careful with my column and don't
ever leave its ferules open, so there is very thin chance of something going
in. My samples of injection are also always filtered thru a 0.22 micron
filter.
Hope to get some answers
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Yoginee Budhkar
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