Dear Yoginee, possibly there is something precipitating on your column (or eluting VERY SLOWLY). Reverse the column and flush it with something like Methanol to get the stuff out.
To extend the life of your column, consider a short "pre-column" (same material, just shorter, a few cm are sufficient) as disposable scavenger for this kind of dirt. HTH Wo _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
