Hey everyone, I'm staining some liver sections with Oil Red O according to a simple protocol I found at IHC world ( http://www.ihcworld.com/_protocols/special_stains/oil_red_o_ellis.htm) and noticed that once I apply the mounting media (Histomount HS103 - http://www.nationaldiagnostics.com/product_info.php/cPath/25_28_35/products_id/188), my stain diffuses out of the tissue. The tissue is definitely stained prior to mounting as it has the characteristic red globule-like lipid dropswhen viewed at x20/x40. Is this due to the fact that Histomount is non-aqueous?
any help much appreciated Chris _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
