I'm a student of Tsinghua University, I'm doing a crosslinking IP recently,
and the crosslinking regent is DSP. My target protein complex is in the
nucleus, however, during my experiment, I found my target protein of the
crosslinked sample even could not be detected in nuclear exact, while the
control sample(i.e., sample without crosslinking) performed well. I tried
different lysis buffer from High Salt solution(350mM NaCl) to RIPA
buffer(0.1%SDS, 1% Triton X-100), so I do wonder what kind of lysis buffer
you use? Thank you!!!
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