Hi, I prefer to use KCl instead of NaCl in your buffer system, it really important play role. In addition, it is better to ensure that your target protein is localised into nucleus before you perform nuclear extraction. I hope this helps you, Good luck.
-- Yours Sincerely, *Naveen Vankadari* ( 那維) Institute of Molecular Biology, ACADEMIA SINICA, Taipei, Taiwan _______________________________________________ Methods mailing list [email protected] http://www.bio.net/biomail/listinfo/methods
