You can use the A260/A280 as an indicator of protein purity. Ideally, pure protein should have an A260/A280 value of 0.6, so 1.05 is very high, and indicates that you have nucleic acid contamination (the A260 component is too high).
________________________________________ From: methods-boun...@oat.bio.indiana.edu [methods-boun...@oat.bio.indiana.edu] on behalf of Sudheer Sangeetham [sudheer.pb...@gmail.com] Sent: Wednesday, October 28, 2015 7:13 AM To: meth...@oat.bio.indiana.edu Subject: the absorbance value 1.05 at 260/280 of protein solution Hello people What does it mean by the absorbance value 1.05 at 260/280 while measuring the protein concentration at 280 nm ? Thank you in advance -- Sudheer Babu.S Research Fellow Institute of Biochemistry Biological Research Center Szeged,Hungary. _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods