Nothing if you are measuring only protein concentration. A Lot if you were measuring DNA concentration. Jay
-----Original Message----- From: methods-boun...@oat.bio.indiana.edu [mailto:methods-boun...@oat.bio.indiana.edu] On Behalf Of Sudheer Sangeetham Sent: Wednesday, October 28, 2015 7:14 AM To: meth...@oat.bio.indiana.edu Subject: the absorbance value 1.05 at 260/280 of protein solution Hello people What does it mean by the absorbance value 1.05 at 260/280 while measuring the protein concentration at 280 nm ? Thank you in advance -- Sudheer Babu.S Research Fellow Institute of Biochemistry Biological Research Center Szeged,Hungary. _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods This email message may contain legally privileged and/or confidential information. If you are not the intended recipient(s), or the employee or agent responsible for the delivery of this message to the intended recipient(s), you are hereby notified that any disclosure, copying, distribution, or use of this email message is prohibited. If you have received this message in error, please notify the sender immediately by e-mail and delete this email message from your computer. Thank you. _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
