Hello, For some reason I had to try and track this down. I might have found enough pieces to let you put it together, not sure.
Here’s the gfp variant, I think: GenBank: U50963.1, https://www.ncbi.nlm.nih.gov/nuccore/1289497/ Here’s Life Technologies’ Focus with a map and some description: Focus 96, Vol. 18, Number 2, https://tools.thermofisher.com/Content/Focus/Focus%20Volume%2018%20Issue%202.pdf And here’s the original reference: Zolotukhin et al. (1996) J. Virol. 70:4646-4654 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC190401/pdf/704646.pdf Then it’s just a matter of tracking down all the plasmid bits and hoping they didn’t make any changes - well, good luck. Back to real work. Barbara MacGregor On 18 Nov 2016, at 12:06, methods-requ...@oat.bio.indiana.edu<mailto:methods-requ...@oat.bio.indiana.edu> wrote: Send Methods mailing list submissions to methods@net.bio.net<mailto:methods@net.bio.net> To subscribe or unsubscribe via the World Wide Web, visit http://www.bio.net/biomail/listinfo/methods or, via email, send a message with subject or body 'help' to methods-requ...@net.bio.net You can reach the person managing the list at methods-ow...@net.bio.net When replying, please edit your Subject line so it is more specific than "Re: Contents of Methods digest..." Today's Topics: 1. Re: pGreen Lantern sequence file (arregui.leti...@gmail.com) 2. Re: pGreen Lantern sequence file (Wolfgang Schechinger) From: <arregui.leti...@gmail.com> Subject: Re: pGreen Lantern sequence file Date: 17 November 2016 12:37:09 GMT-5 To: <methods@net.bio.net> El martes, 5 de marzo de 2002, 21:59:52 (UTC-6), Wolfgang Schechinger escribió: Dear all, Does anyone have sequence file of pGreen Lantern to share? This vector (it's not pGreen for agrobacterium mediated gene transfer to plants) was sold several years ago from Gibco BRL. Thanx! Wo ----- Dr. Wolfgang Schechinger Institute of Biomedical Sciences Academia Sinica, Taipei, Taiwan R.o.C. --- Hi, can you please send me the sequence please, I can´t find it. Thanks, Leticia Arregui Departamento de Ciencias Naturales UAM Cuajimalpa, Mexico From: Wolfgang Schechinger <hubah...@gmx.de> Subject: Re: pGreen Lantern sequence file Date: 17 November 2016 18:41:55 GMT-5 To: <arregui.leti...@gmail.com>, <meth...@magpie.bio.indiana.edu> H Leticia, I don't have it, sorry. Never got the data upon my request. Good luck Wolfgang On 2016年11月18日 01:37, arregui.leti...@gmail.com wrote: El martes, 5 de marzo de 2002, 21:59:52 (UTC-6), Wolfgang Schechinger escribió: Dear all, Does anyone have sequence file of pGreen Lantern to share? This vector (it's not pGreen for agrobacterium mediated gene transfer to plants) was sold several years ago from Gibco BRL. Thanx! Wo ----- Dr. Wolfgang Schechinger Institute of Biomedical Sciences Academia Sinica, Taipei, Taiwan R.o.C. --- Hi, can you please send me the sequence please, I can´t find it. Thanks, Leticia Arregui Departamento de Ciencias Naturales UAM Cuajimalpa, Mexico _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods --------------------------------------------- Barbara MacGregor University of North Carolina Department of Marine Sciences 3117C Venable Hall (a/k/a Murray) CB#3300 Chapel Hill NC 27599 Email bmacg...@unc.edu<mailto:bmacg...@unc.edu> Cell +1 (919) 260-6038 Fax +1 (919) 962-1254 Lab 3110 Venable Hall
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