Hi, I used the information to piece together a candidate for the pGreen Lantern-1 sequence in order to demonstrate the capabilities of pydna. The result is here: http://nbviewer.jupyter.org/github/BjornFJohansson/pydna-examples/blob/master/pGreenLantern1.ipynb
/bjorn On Saturday, November 19, 2016 at 8:22:57 PM UTC, MacGregor, Barbara wrote: > Hello, > > For some reason I had to try and track this down. I might have found enough > pieces to let you put it together, not sure. > > Here’s the gfp variant, I think: GenBank: U50963.1, > https://www.ncbi.nlm.nih.gov/nuccore/1289497/ > > Here’s Life Technologies’ Focus with a map and some description: Focus 96, > Vol. 18, Number 2, > https://tools.thermofisher.com/Content/Focus/Focus%20Volume%2018%20Issue%202.pdf > > And here’s the original reference: Zolotukhin et al. (1996) J. Virol. > 70:4646-4654 > https://www.ncbi.nlm.nih.gov/pmc/articles/PMC190401/pdf/704646.pdf > > Then it’s just a matter of tracking down all the plasmid bits and hoping they > didn’t make any changes - well, good luck. Back to real work. > > Barbara MacGregor > > On 18 Nov 2016, at 12:06, > methods-requ...@oat.bio.indiana.edu<mailto:methods-requ...@oat.bio.indiana.edu> > wrote: > > Send Methods mailing list submissions to > methods@net.bio.net<mailto:methods@net.bio.net> > > To subscribe or unsubscribe via the World Wide Web, visit > http://www.bio.net/biomail/listinfo/methods > or, via email, send a message with subject or body 'help' to > methods-requ...@net.bio.net > > You can reach the person managing the list at > methods-ow...@net.bio.net > > When replying, please edit your Subject line so it is more specific > than "Re: Contents of Methods digest..." > Today's Topics: > > 1. Re: pGreen Lantern sequence file (arregui.leti...@gmail.com) > 2. Re: pGreen Lantern sequence file (Wolfgang Schechinger) > > From: <arregui.leti...@gmail.com> > Subject: Re: pGreen Lantern sequence file > Date: 17 November 2016 12:37:09 GMT-5 > To: <methods@net.bio.net> > > > El martes, 5 de marzo de 2002, 21:59:52 (UTC-6), Wolfgang Schechinger > escribió: > Dear all, > > Does anyone have sequence file of pGreen Lantern to share? > > This vector (it's not pGreen for agrobacterium mediated gene transfer to > plants) was sold several years ago from Gibco BRL. > > Thanx! > > Wo > > ----- > Dr. Wolfgang Schechinger > Institute of Biomedical Sciences > Academia Sinica, Taipei, Taiwan R.o.C. > > --- > > Hi, can you please send me the sequence please, I can´t find it. > Thanks, > Leticia Arregui > Departamento de Ciencias Naturales > UAM Cuajimalpa, Mexico > > > > > From: Wolfgang Schechinger <hubah...@gmx.de> > Subject: Re: pGreen Lantern sequence file > Date: 17 November 2016 18:41:55 GMT-5 > To: <arregui.leti...@gmail.com>, <meth...@magpie.bio.indiana.edu> > > > H Leticia, > > I don't have it, sorry. Never got the data upon my request. > > Good luck > > Wolfgang > > On 2016年11月18日 01:37, arregui.leti...@gmail.com wrote: > El martes, 5 de marzo de 2002, 21:59:52 (UTC-6), Wolfgang Schechinger > escribió: > Dear all, > > Does anyone have sequence file of pGreen Lantern to share? > > This vector (it's not pGreen for agrobacterium mediated gene transfer to > plants) was sold several years ago from Gibco BRL. > > Thanx! > > Wo > > ----- > Dr. Wolfgang Schechinger > Institute of Biomedical Sciences > Academia Sinica, Taipei, Taiwan R.o.C. > > --- > Hi, can you please send me the sequence please, I can´t find it. > Thanks, > Leticia Arregui > Departamento de Ciencias Naturales > UAM Cuajimalpa, Mexico > _______________________________________________ > Methods mailing list > Methods@net.bio.net > http://www.bio.net/biomail/listinfo/methods > > > > > _______________________________________________ > Methods mailing list > Methods@net.bio.net > http://www.bio.net/biomail/listinfo/methods > > --------------------------------------------- > Barbara MacGregor > University of North Carolina > Department of Marine Sciences > 3117C Venable Hall (a/k/a Murray) > CB#3300 > Chapel Hill NC 27599 > > Email bmacg...@unc.edu<mailto:bmacg...@unc.edu> > Cell +1 (919) 260-6038 > Fax +1 (919) 962-1254 > Lab 3110 Venable Hall _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
