Dear sir, We check the fermentation broth at intervals and do not see any contamination. When we homogenise (1000 bar / 3 pass) this cell mass we do get our protein. But due to this slimy nature it becomes difficult to prepare a homogenous cell paste suspension.
thanks and regards, megha On Thu, Nov 9, 2017 at 8:43 PM, Wolfgang Schechinger <hubah...@gmx.de> wrote: > Looks like contamination, Megha. Do you find your protein in the slime? > > Regards, > Wo > > > On 09.11.2017 14:11, Megha Goyal wrote: > >> Dear All, >> >> We are doing fermentation for recombinant protein using E.coli culture. >> Earlier we used to obtain compact cell pellet on harvesting (3 Hrs after >> IPTG Induction). But recently we observed that the cell pellet harvested >> are of slimy consistency and they do not homogenise in the washing buffer >> (tris-nacl-edta). We observe thread like consistency and no matter of >> stirring it do we get a homogeneous suspension. >> >> Can someone guide me what is the reason for such phenomenon. >> >> Thanks >> >> megha >> _______________________________________________ >> Methods mailing list >> Methods@net.bio.net >> http://www.bio.net/biomail/listinfo/methods >> > > _______________________________________________ Methods mailing list Methods@net.bio.net http://www.bio.net/biomail/listinfo/methods
