For some new applications we are doing sequencing where many samples are multiplexed in the same library. I'm wondering if there is a de facto standard for how to represent multiplexed samples in a SAM file.
According to the specification SM or @RG should "use pool name where a pool is being sequenced". Is LB of @RG assumed to be a globally unique identifier (or unique within the SAM file) to indicate that the different samples are from the same library? Do most of the popular tools interpret these headers in a uniform way? Thanks, -Bob ------------------------------------------------------------------------------ _______________________________________________ Samtools-help mailing list [email protected] https://lists.sourceforge.net/lists/listinfo/samtools-help
