[Histonet] I have another question for the collective

[P Sicurello via Histonet]

Good Afternoon Listers,

Here's a question for those who work in the academic clinical arena.
Background:  We have researchers who have IRBs in place.  They sign a
patient up for their study.  The researcher wants slides cut on patient
tissue to use for their study.  They want it right away (of course).

Question:  Do you charge them extra when they want their slides
rushed?   When I worked in research (almost 10 years ago) I
charged extra for rush cases:  $50 for next day, $100 for same day.

Thanks in advance!

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

9300 Campus Point Drive

San Diego, CA 92037

(P): 858-249-5610



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Re: [Histonet] Elephant Tissues

[P Sicurello via Histonet]

Jennifer,

I have worked on mouse, rat, rabbit, sea lion, harbor seal, killer whale,
giraffe, and even human mummy tissues.  With the exception of the mummy
tissue being a bit dry, they all embedded and cut like human tissue.

What is it that is making them hard to cut?

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



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On Wed, Sep 5, 2018 at 10:38 AM Jennifer Phinney via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

> Hello Histonetters,
> Does anyone have experience processing and cutting elephant tissues?  Any
> tips, tricks, or advice?  My lab has had some elephant cases recently and
> the tissues are unexpectedly (to us) difficult to cut.
>
> Thanks for any help,
> Jennifer Phinney QIHC
> Kansas State University
> Veterinary Diagnostic Lab
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> Histonet@lists.utsouthwestern.edu
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>
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Re: [Histonet] Unstained slides

[P Sicurello via Histonet]

How about frozen sections cut for immunofluorescence stored at -20?

Sincerely,

Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



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On Wed, Sep 5, 2018 at 1:12 PM Hobbs, Carl via Histonet <
histonet@lists.utsouthwestern.edu> wrote:

>
> Hi
>
> Depends on what you mean by cryosections.
> Unfixed/fixed?
> Stored at RT, 4C, -20C, -80C.
> Stored dry or in glycerol
>
> So many variables!
> My opinion is to store blocks and cut sections as required.
> Least variables.
> Sure, one loses some tissue everytime one cuts anewa good thing.
>
> It IS complicated so, a project has to be thought out well in advance.
>
> Stimulating post
>
>
>
> Carl Hobbs FIBMS
> Histology and Imaging Manager
> Wolfson CARD
> Guys Campus, London Bridge
> Kings College London
> London
> SE1 1UL
>
> 020 7848 6813
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[Histonet] Alcian Blue stain on lab coats

[Eileen Akemi Allison via Histonet]

Hello Histo Peeps:

I have two new lab coats which have been stained with Alcian Blue and can’t get 
the stains out.  I have tried oxy clean incorporated with bleach, as well as 
pre-soaking in 3% acetic acid, and American Mastertech’s stain remover to no 
avail.  Any suggestions would be greatly appreciated.

Thank you in advance,

Akemi Allison, BS, HT/HTL (ASCP)
Pathology Manager
Monterey Bay GI Consultants Laboratory
23 Upper Ragsdale Drive, Suite 200
Monterey, CA 93940
W: Email: aalli...@montereygi.com 
H: Email: akemiat3...@gmail.com 
Tele: (831) 375-3577 X117
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Re: [Histonet] Unstained slides

[Hobbs, Carl via Histonet]

Hi

Depends on what you mean by cryosections.
Unfixed/fixed?
Stored at RT, 4C, -20C, -80C.
Stored dry or in glycerol

So many variables!
My opinion is to store blocks and cut sections as required.
Least variables.
Sure, one loses some tissue everytime one cuts anewa good thing.

It IS complicated so, a project has to be thought out well in advance.

Stimulating post


 
Carl Hobbs FIBMS 
Histology and Imaging Manager 
Wolfson CARD 
Guys Campus, London Bridge  
Kings College London 
London 
SE1 1UL 
  
020 7848 6813
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Re: [Histonet] Temperature for Storing Slides and Blocks

[WILLIAM DESALVO via Histonet]

I do not know of a standard, but you should make sure the storage temp is 5-10 
degrees lower(maybe more depending on fluctuations in temp for the area). That 
would be < 120F degrees. Request the location Facilities department understands 
you need temperature control

William DeSalvo


From: Dessasau III, Evan via Histonet 
Sent: Wednesday, September 5, 2018 11:52 AM
To: Blake Taylor
Cc: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] Temperature for Storing Slides and Blocks

When we had our structure built we had cooling/heating units(2) put in a 
structure that is about 818sqft. 70F summer and 65 to 68F winter is what we aim 
for. Not sure if that is ideal but it keeps the blocks cool in the summer and 
the slides from sticking in the winter.
Thank you,
E-van
Histology Lab, Yerkes
Rm. 2122
7-7744 Lab
7-7902 office



-Original Message-
From: Blake Taylor via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, September 5, 2018 1:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Temperature for Storing Slides and Blocks

Is there a good reference or standard on what the room temperature should be 
for storing slides and blocks? We have moved our long term storage out to our 
hospitals warehouse and I believe the building is getting much too hot. What 
temperature range is everyone using?

Thanks so much

Blake Taylor
Surgical Pathology Supervisor
Lexington Medical Center
803-936-8214
bcdu...@lexhealth.org

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Re: [Histonet] Temperature for Storing Slides and Blocks

[Dessasau III, Evan via Histonet]

When we had our structure built we had cooling/heating units(2) put in a 
structure that is about 818sqft.  70F summer and 65 to 68F winter is what we 
aim for.  Not sure if that is ideal but it keeps the blocks cool in the summer 
and the slides from sticking in the winter.
Thank you,
E-van
Histology Lab, Yerkes
Rm. 2122
7-7744 Lab
7-7902 office



-Original Message-
From: Blake Taylor via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, September 5, 2018 1:53 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Temperature for Storing Slides and Blocks

Is there a good reference or standard on what the room temperature should be 
for storing slides and blocks?  We have moved our long term storage out to our 
hospitals warehouse and I believe the building is getting much too hot.  What 
temperature range is everyone using?

Thanks so much

Blake Taylor
Surgical Pathology Supervisor
Lexington Medical Center
803-936-8214
bcdu...@lexhealth.org

PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are 
confidential property of the sender. The information is intended only for the 
use to the person to whom it was addressed. Any other interception, copying, 
accessing, or disclosure of this message is prohibited. The sender takes no 
responsibility for any unauthorized reliance on this message. If you have 
received this message in error, please immediately notify the sender and purge 
the message you received. Do not forward this message without permission.
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[Histonet] Temperature for Storing Slides and Blocks

[Blake Taylor via Histonet]

Is there a good reference or standard on what the room temperature should be 
for storing slides and blocks?  We have moved our long term storage out to our 
hospitals warehouse and I believe the building is getting much too hot.  What 
temperature range is everyone using?

Thanks so much

Blake Taylor
Surgical Pathology Supervisor
Lexington Medical Center
803-936-8214
bcdu...@lexhealth.org

PRIVILEGED AND CONFIDENTIAL: This electronic message and any attachments are 
confidential property of the sender. The information is intended only for the 
use to the person to whom it was addressed. Any other interception, copying, 
accessing, or disclosure of this message is prohibited. The sender takes no 
responsibility for any unauthorized reliance on this message. If you have 
received this message in error, please immediately notify the sender and purge 
the message you received. Do not forward this message without permission.
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[Histonet] Elephant Tissues

[Jennifer Phinney via Histonet]

Hello Histonetters,
Does anyone have experience processing and cutting elephant tissues?  Any tips, 
tricks, or advice?  My lab has had some elephant cases recently and the tissues 
are unexpectedly (to us) difficult to cut.

Thanks for any help,
Jennifer Phinney QIHC
Kansas State University
Veterinary Diagnostic Lab
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