Re: [ccp4bb] AW: [ccp4bb] Phosphatase enzymatic assay

Dear all, Thanks a lot for all your nice suggestions, I really appreciate them. I am now digging through all the methods you suggest trying to find the one that will solve my enzimatic issue. For the ones that asked what was not working, I am trying to replicate some data but with not much succ

[ccp4bb] PhD position in Vienna, Austria (University of Natural Resources and Life Sciences)

Dear colleagues, there is an opening for a PhD position in the Department of Chemistry, University of Natural Resources and Life Sciences, Vienna, Austria. The work will have a strong focus on structural biology methods, investigating a highly important enzyme involved in prokaryotic heme biosy

[ccp4bb] Postdoc position at University of Colorado School of Medicine

To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www

[ccp4bb] Two postdoc positions in Newcastle University on structural biology & drug discovery

Dear all The Yue lab has recently moved to the Newcastle University Biosciences Institute, and is now up and running! www.staff.ncl.ac.uk/yuelab/ We are currently recruiting two postdoc positions in structure-guided drug discovery. 1. Want to work on a hit-to-lead translational project i

[ccp4bb] PDB file secondary structure annotations

Dear All, I noticed that files available in the Protein Data Bank contain secondary structure information which allows for them to be imported in a program such as BioRender in order to make structural figures. However, the PDB files generated by PHENIX for structures that I haven't deposited yet

[ccp4bb] Re-Release of iMosflm version 7.4.0

Dear All, Version 7.4.0 of iMosflm has just been re-released and can be downloaded from: https://www.mrc-lmb.cam.ac.uk/mosflm/imosflm/ver740/introduction.html *** Note that the Windows version will not process HDF5 images (

Re: [ccp4bb] COOT running on Macbook Pro M1 chip

It helps :) Thanks. Wim On Thu, 16 Sept 2021 at 20:40, Jon Cooper < 488a26d62010-dmarc-requ...@jiscmail.ac.uk> wrote: > Maybe turning off smooth re-centering would help our mac-using friends ;-? > > > Sent from ProtonMail mobile > > > > Original Message > On 16 Sep 2021, 11

[ccp4bb] AW: [ccp4bb] Phosphatase enzymatic assay

It may also be possible to separate the different phosphorylated forms on an isoelectric focusing gel. Best, Herman Von: CCP4 bulletin board Im Auftrag von Patrick Loll Gesendet: Mittwoch, 15. September 2021 15:12 An: CCP4BB@JISCMAIL.AC.UK Betreff: Re: [ccp4bb] Phosphatase enzymatic assay Phos-

Re: [ccp4bb] Double covalent ligand

Dear Rens, CCP4i2 now contains a CIF dictionary accumulator, which should help with handling such problems (note it's also available as standalone to be used via command line if that's useful). This is used automatically when providing multiple ligand CIF dictionaries to the Refinement interfac

[ccp4bb] Research Scientist and Postdoctoral Fellow positions available at University of Texas Health Science Center San Antonio

My research group at the University of Texas Health Science Center, San Antonio, is seeking to hire a Research Scientist and a Postdoctoral Fellow to study the structures and function of RNA modification and chromatin remodeling enzymes. We will use X-ray crystallography, cryo-EM, and enzyme bioche

[ccp4bb] Double covalent ligand

Dear all, I was wondering if there was a good way to generate a restraint file for ligands that are covalently bound to two sites of the same protein. Just to clarify, the ligand can be viewed as a "bridge" between two residues of the same protein monomer. At present, I use JLigand to prepare

[ccp4bb] PSDI 2021 Virtual Conference ****Registration Open**** Nov 8th-10th, 2021

Dear Colleagues, Following our recent announcement advising you that this year’s PSDI 2021 Conference would be held virtually (Nov 8^th -10^th , 14:00 pm– 18:00 pm CET). We are now delighted to invite you to register and sign up for the conference. This year the conference is organized by Me

Re: [ccp4bb] COOT running on Macbook Pro M1 chip

On 16/09/2021 08:40, WENHE ZHONG wrote: Dear CCP4 community, The COOT is not running smoothly on my M1 chip Macbook. For example, when both model and the electron density map are displayed, the moving from one residue to the next (pressing SPACE bar) is lagging/slow (>2s). This only happened

Re: [ccp4bb] COOT running on Macbook Pro M1 chip

Maybe turning off smooth re-centering would help our mac-using friends ;-? Sent from ProtonMail mobile Original Message On 16 Sep 2021, 11:29, Weiergräber, Oliver H. wrote: > Hi, > > You may want to check the number of "steps" used in re-centring. > This setting strongly affect

[ccp4bb] Job posting: Senior Structural Biologist (X-Ray Data Collection) at Helix BIostructures LLC

Dear CCP4BB, Helix Biostructures is looking for a talented Scientist / Senior Scientist with experience in structural biology, specifically synchrotron X-Ray data collection and protein structure solution. The ideal candidate would have a substantial background in X-ray data collection or a gener

[ccp4bb] Open Position: Beamline Scientist - HZB BESSY II in Berlin, Germany

Postdoc/Beamline Scientist (m/f/d) Reference No.: PS 2021/9 The Macromolecular Crystallography (MX) group at the HZB is operating and developing three beamlines for MX at the BESSY II storage ring for the national and international user community. The MX-group is also in charge of the HZB-MX-Bi

Re: [ccp4bb] COOT running on Macbook Pro M1 chip

Hi, You may want to check the number of "steps" used in re-centring. This setting strongly affects the perceived speed ;-) Best regards Oliver == PD Dr. Oliver H. Weiergräber Institut für Biologische Informationsprozesse IBI-7: Strukturbioche

Re: [ccp4bb] COOT running on Macbook Pro M1 chip

Hi Claude, Pymol 2.4.0 (installed via homebrew) works fine for me in Apple M1 MacBook Pro, 2020. Ciao, S > On 16 Sep 2021, at 12:12, Claude Sauter wrote: > > Dear all, > > to continue on the same theme, a colleague biochemist asked me a couple of > days ago if there is any incompatibility bet

Re: [ccp4bb] COOT running on Macbook Pro M1 chip

Dear Wim, on my M1 Macbook Air, coot 0.9.6 (latest from CCP4) works smoothly/fast. What is your XQuartz version? I have 2.8.1 . HTH, Kay On Thu, 16 Sep 2021 15:40:53 +0800, WENHE ZHONG wrote: >Dear CCP4 community, > >The COOT is not running smoothly on my M1 chip Macbook. For example, when >

Re: [ccp4bb] COOT running on Macbook Pro M1 chip

Dear all, to continue on the same theme, a colleague biochemist asked me a couple of days ago if there is any incompatibility between PyMOL and other softwares used in structural biology and new mac M1 chips. Being a Linux user, I

[ccp4bb] COOT running on Macbook Pro M1 chip

Dear CCP4 community, The COOT is not running smoothly on my M1 chip Macbook. For example, when both model and the electron density map are displayed, the moving from one residue to the next (pressing SPACE bar) is lagging/slow (>2s). This only happened to my old computer, but I am surprised to fin

Re: [ccp4bb] Phosphatase enzymatic assay

Hi, Could you radiolabel the kinase? If yes, measuring the labeled Pi may work. Cheers, Seema To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1