Good Morning,
we are interested in replacing xylene in our lab. Is anyone using graded
isopropyl alcohols in there processors instead of xylene and if so how is it
working for you? Thanks.
Jessica Piche-Grocki, HT(ASCP)
Waterbury Hospital
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Announcing MDS Analytical Technologies next in the series of Laser
Capture Microdissection and Microgenomics Webinars. Our next LCM
webinar will be held on Monday, November 24, 2008 at 10AM PST or 1PM
EST. The speaker will be Charmain Pietersen, PhD., Laboratory for
Structural and Molecular
And... How many of us have developed sensitivity to d-limonene? I for one
have a sensitivity to it and more and more Mohs labs are forgoing fume hoods
because it is considered 'safe'.
Mickie
Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
Mohs Lab Staffing
Mohs
I am searching for a used tissue processor in good condition.
Please contact me by e-mail:
[EMAIL PROTECTED]
Cindy DuBois
Integrated Pathology
Stockton, Ca
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Are the coplin jars all equally filled? If the wash-jars are partly filled,
the lower slides would be more differentiated than the upper.
Gudrun
-Ursprüngliche Nachricht-
Von: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] Im Auftrag von Karla
Arrington
Gesendet: Mittwoch, 19. November 2008
Jodie,
That's for CD4 not C4d
Ronnie Houston
Anatomic Pathology Manager
Nationwide Children's Hospital
Columbus OH 43205
(614) 722 5450
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Jodie
Robertson
Sent: Wednesday, November 19, 2008 9:34 AM
To: Richard
You have to stress the first and the third syllable.
Like sun-shine-rag-gy Tell-yes-nicz-ky
Gudrun
-Ursprüngliche Nachricht-
Von: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] Im Auftrag von Robert
Richmond
Gesendet: Mittwoch, 19. November 2008 02:53
An: histonet@lists.utsouthwestern.edu
We are using the Benchmark XT. Sorry I omitted that.
Susan M Weber HT(ASCP)
Histology Supervisor
Louis Stokes Cleveland VA Medical Center
10701 East Blvd
Cleveland, Ohio 44106
(216) 791-3800 X6154
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] On Behalf Of Jesus
We are using the ultraView DAB from Ventana and the same HSA from Cell
Marque. Here is the protocol we are using. Depar as usual; CC1 Standard;
HSA incubation at 37C for 32 min; Counterstain Heme 4 min; Post
Counterstain Bluing for 4 min. we have found it to be a reliable stain
and very clean and
R. Tellyesniczky published more than one
fixative,nbsp;1898-190= 5. His best-known one translates into
AFA or FAA: mostly ethyl alcoh= ol, with about 4% formaldehyde
and about 5% acetic acid. It was = reinvented in 1937 by David
Bodian (EMAnat. Rec/EM.
All ThermoWave Microwave Processing users
Would any of you be filling to share your needle cor biopsy SOP (i.e. prostate)
and the GI SOP?
Thanks
Roxanne
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Sounds to me that your having a de-paraffining problem. Check your xylene or
clearing agent, maybe there is water in them or they are not as clean as they
should be. Are you on a daily rotation/change of staining/de-paraffin
reagents? Maybe, your oven isn't hot enough to melt some paraffin
PIPES-buffered glutaraldehyde has been used for subsequent electron
micr= oscopy since the early 1970s but it has been shown to cause
artifacts- = intracellular vesicles and myelin figures
-nbsp;when used for perfu= sion fixation of the brain. See
FONT
Hi All,
I am wondering if anyone has experience showing therapeutic monoclonal antibody
binding in mouse xenografts (human tumor cells injected subcutaneously in SCID
mice and then dose treated with therapeutic antibody injected
intraperitoneally). For instance, can trastuzamab (Herceptin)
We have used an automated special stainer for years and are now experiencing
multiple problems. Therefore before we order from the same vendor I wondered
what everyone else was using for silver stains such as Jones, Retic, and GMS.
Just trying to evaluate if replacing instrument with newer
We had a problem with our recycler and xylene contamination so we now
test each batch by adding water to a sample of recycled alcohol. If it
turns cloudy we assumed that we had xylene contamination.
We have lately tested commercial ethanols (no turbidity)before placing
them on the processor and
Just another FYI...
The Biocare IntelliPath does the same thing, but has 4 trays of 10 and it
allows you to make one of the trays?RUSH.? SO depending, on the volume?of IHC
you do either of these machines would work out great.
Roxanne
-Original Message-
From: Josie Britton
Hi Claire
Try this company, Microscope Mictorome Service Company. I use them and
they are very good. Fred Siena - Tissue Techniques Pathology Labs
Click to consolidate debt and lower month expenses.
I didn't think that it had deparaffinization and retrieval on line.
-Original Message-
From: [EMAIL PROTECTED]
[mailto:[EMAIL PROTECTED] Behalf Of
[EMAIL PROTECTED]
Sent: Wednesday, November 19, 2008 5:18 PM
To: [EMAIL PROTECTED]; [EMAIL PROTECTED];
Histonet@lists.utsouthwestern.edu
I am sorry---it is 5 trays of 10 including the RUSH tray
Roxanne
-Original Message-
From: [EMAIL PROTECTED]
To: [EMAIL PROTECTED]; [EMAIL PROTECTED]; Histonet@lists.utsouthwestern.edu
Sent: Wed, 19 Nov 2008 5:17 pm
Subject: Re: [Histonet] Dako
Just another FYI...
The Biocare
Hello Everyone,
I have been following Victor's updates on the PSlim slide printer the past
couple of months.
Who else is using this device out there?
I have some questions about it.
What kind of slides are you using?
Have you had any issues with it?
Thanks in advance,
Tyler Liebig
[EMAIL
Histonet,
anybody know where I can find Sheep Liver, we have a research person looking
for a small piece or a block.
Thanks in advance.
Rudy
[EMAIL PROTECTED]
Methodist. Leading Medicine.
Ranked No. 10 on FORTUNE magazine's list of the 100 Best Companies to Work
For in 2008
One other possiblity to consider is a thinner section at the bottom. That
could be the answer if the problem is intermittent even within the same
staining rack. If the section is thinner, the staining appears paler.
Good luck discovering the cause!
We had the same problem. The xylene had nothing to do with it and thick and
thin sections was one thing that we thought might be the problem. But when I
destained and restained the same slides they stained the way they were supposed
to . Finally we found that we had a problem with the wash
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