Ask PolySciences. If they make the mounting medium thay must know what
dissolves it. Please pass on their reply to histonet@lists.utsouthwestern.edu
John Kiernan
Anatomy, UWO
London, Canada
= = =
- Original Message -
From: Michele Wich
Date: Wednesday, December 2, 2009 17:07
Subject: [H
we go live tomorrow night!!!
AP all set up and good to go
AbuDhabiAnnie
2009/12/2 Evans, Andria B
> Does anyone out there in Histoland use Cerner Millenium in there lab? I
> have some questions if you could contact me that would be great.
> Thanks!
>
> Andria B Evans, HTL(ASCP)CM
> Anatomic Pat
No
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA
-Original
Does anyone know if you can substitute hydrochloric acid for phosphomolybdic
when performing a masson trichrome stain?
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Hi all
to stain out the trophic factors on brain sections, such as BDNFm, IGF1, NGF,
CNTF, GDNF
will these molecules "leak" out during staining? so I should not use triton and
try to shorten the time of staining?
Or I need a long time incubation to stain out the "low" concentration small
molecu
Hi,
Yes, of course it's dangerous. (OK, granted really dangerous) Histology
cannot be done with distilled water alone. Risks are part of the job.
Knowledge of the risks and understanding of how to minimize these risks is
what makes it possible to do our jobs and not have serious problems.
When I first entered the field of histotechnology, I was told you needed on the
job training or an Associate's degree for a HT and a Bachelors for a HTL. Yet
so many ads nowadays state you MUST have a B.S. degree in science for a HT
position.
Like I had written earlier in the year, I am trying
Hello. Can anyone tell me how I might remove coverglass from slides
containing GMA sections mounted with "UV mount" (PolySciences)?
Thank you.
This communication is intended solely for the use of the addressee and may
contain information that is legally privileged, confidential or exempt from
Because those whose SS# are for everybody to see do not know!
René J.
--- On Wed, 12/2/09, Sebree Linda A wrote:
From: Sebree Linda A
Subject: RE: [Histonet] CAP question
To: "Rene J Buesa" , "JoyceWeems" ,
histonet@lists.utsouthwestern.edu, "AnnaInman"
Date: Wednesday, December 2, 2009, 4:3
Dear Histonetters
Our laboratory has advertised for a registered HT or HTL (ASCP) and the
following job description is posted on our university website. Although MS
degree was put as the required qualification, a bachelors degree will be
closely considered. Bozeman is located 90 miles nort
Leads me to wonder how the VA gets away with using SS#s as identifiers!
Linda A. Sebree
University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
DB1-223 VAH
600 Highland Ave.
Madison, WI 53792
(608)265-6596
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto
Hi JoAnne,
Our pathologist requested we try a Faulkner stain for Mycoplasma. I found two
methods. The Faulkner/Lillie and the Faulkner/Kerr variations of the Warthin
Starry. One of these is a lower acid. The pathologist is looking up the
reference and will call the people who wrote a paper o
I would strongly suggest not using the SS# as a second identifier. That is a
piece of information the patient gave to the hospital but I am sure the patient
would not like to see written in a cassette/slide for all to see (and copy).
René J.
--- On Wed, 12/2/09, Inman, Anna wrote:
From: Inman
I really would suggest you to find an alternative procedure.
Osmium tetroxyde is EXTREMELY dangerous and has to be used under extreme
precautions.
I don't think the results are worth the effort given the great dangers it poses.
I for one would not share the procedure with you.
René J.
--- On Wed,
OSMIUM TETROXIDE FOR FAT (Paraffin SECTIONS)
Solutions
2.5% Potassium Dichromate Solution Stock
1% Osmium Tetroxide Solution Stock
Potassium Dichromate-Osmium Tetroxide Solution Working
Potassium Dichromate Stock 50 ml
Osmium Tetroxide stock 50 ml
Staining Procedure
1. Place formalin f
Light Micro. I have done this before and it turned out great on some
mouse muscle, but I misplaced my procedure.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, Colorado 80308
office (303) 682-3949
fax (303) 682-9060
www.premierlab.com
Ship
Dalton's Chrome-osmium fix was popular for awhile in the 1950's. You
will need to look in an older methods book. The combo of dichromate and
osmium will not make your safety officer happy. There are many ways to
stain lipids with osmium, alone or mixed with something else. Is this
for EM or LM?
I'm trying to find a procedure for staining fat in tissues with osmium
and I think potassium dichromate, does anyone out there have this
protocol available. I have found it before on the web but for some
reason I can't find it this time.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manag
Yes, patient name, DOB or SS# and the case number and such is "extra"
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems,
Joyce
Sent: Wednesday, December 02, 2009 10:41 AM
To: histonet@lists.utsouth
As far as I know, a 4 years degree is necessary to be licensed as Supervisor in
Florida.
René J.
--- On Wed, 12/2/09, histospr...@aol.com wrote:
From: histospr...@aol.com
Subject: [Histonet] FL Supervisor License
To: histonet@lists.utsouthwestern.edu
Date: Wednesday, December 2, 2009, 12:59 P
We have a policy which includes a list of tissues that are not required to
be sent to the lab as well as a list of gross only specimens. This
policy is presented at the Medical Staff meeting and is approved in that
committee. Changes are made through the same committee. The Laboratory
Medi
In our lab we have set hours for each histotech and the duties rotate weekly.
The hours we have are
4:30, 5:00, 5:30, 6:00, 6:30, 7:00, 7:30, 8:15, 8:30(x2)
Each time slot has 1 histotech that comes in at the times above except the 8:30
slot, that has 2 people.
If you have any other q
I stand corrected! Mike from Erie (Thermofisher) let me know. You may want
to give him a call...see e-mail below.
"I saw your Cover Glass e-mail on the Histonet, and wanted to let you know
that we can do any custom sizes of cover glass.
We can make rectangles, squares & circles to any specificati
I believe your surgical affairs committee can compose this list.
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR 72202
phone 501.364.4240
fax501.364.3155
visit us on the web at:www.archildren
This may have been covered already, please accept my apologies for a repeat
question. I was curious if anyone knows if you can upgrade a technologist (FL
state license only, grandfathered in so NO ASCP) license WITHOUT a 4 year
degree? The work experience is 10+ years but this person does NOT h
Don't we still need to follow through with two identifiers tho?
From: Inman, Anna [mailto:anna.in...@stmarygj.org]
Sent: Wednesday, December 02, 2009 12:16
To: Lois Anderson; Weems, Joyce; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] CAP question
That's what we use as well. Patient name and DOB
Kim Donadio
Pathology Supervisor
Baptist Hospital
1000 W Moreno St.
Pensacola FL 32501
Phone (850) 469-7718
Fax (850) 434-4996
-
All electronic data transmissions originating from or sent to
Baptist Health
JCAHO standard is patient name and date of birth for the 2 unique identifiers.
Becky Garrison
Pathology Supervisor
Shands Jacksonville
904-244-6237, phone
904-244-4290, fax
904-393-3194, pager
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@l
Keep in mind:
The requirement states 2 patient identifiers at the time of collection
- so the identifiers need to come from the outside entities.
My conversation with CAP - they are going for patient name, DOB, SS# or
medical record number of some sort that ties the hospital to the
clinician
Name and case number or med records number
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
Sent: Wednesday, December 02, 2009 12:00 PM
To: histonet@lists.utsouthwestern.edu
Subject: RE: [Histon
Case number and name
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Una McGiven
Sent: Wednesday, December 02, 2009 11:50
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] CAP question
Hi every
WashingtonLifeScience.com - Career Opportunities
http://www.washingtonlifescience.com/career/career-opp.htm
http://www.washingtonlifescience.com/showjob?jobid=5763
Seattle Children's Hospital
Histology Technician
JOB FUNCTION: Histology
JOB DESCRIPTION:
We invite you to bring your career to an
Hi everyone,
On the most recent CAP checklist there is a requirement for 2 patient
identifiers. For those labs who are CAP and receive all or a large portion of
their cases from outside facilities (=not from your facility), what are you
using as the identifiers on your specimen containers? N
I'm looking for a special stain procedure for mycoplasma. Would the
modified (low pH) Warthin-Starry method work? Thanks,
JoAnne
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Hi All,
There is a new standard from JCAHO addressing which tissue specimens require
only a macroscopic examination. Does anyone have a list of specimens that fit
this standard. We are in the process of writing up a policy for this and would
appreciate your help.
Thanks!
Michele Margiotta
BM
I was told that they would not extend annual maintenance agreements to my
models older than 5 years, but they will come to service older models on a
repair or emergency call/fee-for-service basis.
Jan Shivers
- Original Message -
From: "McMahon, Loralee A"
To: "Xipamanine Mkuze" ;
good morning!! just wanted to make sure about the number of slides that are
required for validation for her 2. is it 25? thanks so much,
anita dudley
providence hospital
mobile alabama
___
Hi all
to stain out the trophic factors on brain sections, such as BDNFm, IGF1, NGF,
CNTF, GDNF
will these molecules "leak" out during staining? so I should not use triton and
try to shorten the time of staining?
Or I need a long time incubation to stain out the "low" concentration small
molecu
The largest production coverslip that I have heard of is a 25mm square
Ken Marissael
VWR Clinical Account Representative
ken_mariss...@vwr.com
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I just renewed my service contract for my autostainer which is 6 years old -
no problem. Just an FYI. Amy
Amy S. Porter, HT (ASCP) QIHC
Investigative HistoPathology Laboratory - Supervisor
2201 Biomedical Physical Sciences Bldg. Rm #2133
East Lansing, MI 48824-3320
Phone: (517) 884-5026
Fax:
Does anyone out there in Histoland use Cerner Millenium in there lab? I have
some questions if you could contact me that would be great.
Thanks!
Andria B Evans, HTL(ASCP)CM
Anatomic Pathology
York Hospital
1001 S. George Street
York, PA 17405
717-851-5006
"You can learn a lot more from liste
Walter,
I'm just curious - how many pathologists do you have? How many tissue
processors? and what is your average TAT?
Thanks,
Toni
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]on Behalf Of Walter
Benton
Sent: Wedn
Brain Research Laboratories has anything up to 4"x 5". We use them for monkey
slides.
Individuals who have received this information in error or are not authorized
to receive it must promptly return or dispose of the information and notify the
sender. Those individuals are hereby notified tha
We have fixed tech schedules, but we rotate task within the lab weekly to keep
everyone fresh and competent.
Our shifts include
4am (3)
6am (2)
7am (2)
8am (1)
Walter Benton, HT(ASCP)QIHC
Histology Supervisor
University of Maryland Medical Center
Anatomic Pathology
22 S. Greene St
Room NBW
Dako would you respond to this issue?
Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
1 Children's WaySlot 820
Little Rock, AR 72202
phone 501.364.4240
fax501.364.3155
visit us on the web at:www.archildrens.org
-Original Messa
I think that they are not supporting the older model of autostainer. They are
still selling a new version of the autostainer.
Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
_
Dako will no longer support Autostainers? Why?
Ninni
I need to pick your collective brains here about getting a service contract
for
my Dako Autostainers.
I remember seeing a posting awhile back with the name of a company, I think
from MD, that will service the Dako Autostainers now that Dako wil
Fisher Scientific carries 50x35 mm and 50x45 mm coverglass.
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Hello All,
We want to do a 3D reconstruction from serial histology samples. On the we I
have found Amira, BioVis 3D and Surf Driver software. If anyone could offer any
input or recommend one of these program it would be greatly appreciated. If
there are any open source programs that could be u
we use the cell signaling one. PCNA of mouse IgG
2009-12-02
TF
发件人： Richard J
发送时间： 2009-12-02 19:04:06
收件人： linresearch
抄送： histonet
主题： Re: [Histonet] PCNA
Looks like clone PC10 works well in rat, mouse, human, chick and zebrafish
Protocols and images at
http://www.immunoportal.
Looks like clone PC10 works well in rat, mouse, human, chick and zebrafish
Protocols and images at
http://www.immunoportal.com/modules.php?name=gallery2&g2_view=keyalbum.KeywordAlbum&g2_keyword=PCNA
2009/12/2
>
>
> Hello,
> Can anyone recommend PCNA antibody that works in FFPE rat tissue?
> Woul
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