Good Afternoon Listers,
Here's a question for those who work in the academic clinical arena.
Background: We have researchers who have IRBs in place. They sign a
patient up for their study. The researcher wants slides cut on patient
tissue to use for their study. They want it right away (of
Jennifer,
I have worked on mouse, rat, rabbit, sea lion, harbor seal, killer whale,
giraffe, and even human mummy tissues. With the exception of the mummy
tissue being a bit dry, they all embedded and cut like human tissue.
What is it that is making them hard to cut?
Sincerely,
Paula
How about frozen sections cut for immunofluorescence stored at -20?
Sincerely,
Paula Sicurello, HTL (ASCP)CM
Histotechnology Specialist
UC San Diego Health
200 Arbor Drive
San Diego, CA 92103
(P): 619-543-2872
*Confidentiality Notice*: The information transmitted in this e-mail is
Hello Histo Peeps:
I have two new lab coats which have been stained with Alcian Blue and can’t get
the stains out. I have tried oxy clean incorporated with bleach, as well as
pre-soaking in 3% acetic acid, and American Mastertech’s stain remover to no
avail. Any suggestions would be greatly
Hi
Depends on what you mean by cryosections.
Unfixed/fixed?
Stored at RT, 4C, -20C, -80C.
Stored dry or in glycerol
So many variables!
My opinion is to store blocks and cut sections as required.
Least variables.
Sure, one loses some tissue everytime one cuts anewa good thing.
It IS
I do not know of a standard, but you should make sure the storage temp is 5-10
degrees lower(maybe more depending on fluctuations in temp for the area). That
would be < 120F degrees. Request the location Facilities department understands
you need temperature control
William DeSalvo
When we had our structure built we had cooling/heating units(2) put in a
structure that is about 818sqft. 70F summer and 65 to 68F winter is what we
aim for. Not sure if that is ideal but it keeps the blocks cool in the summer
and the slides from sticking in the winter.
Thank you,
E-van
Is there a good reference or standard on what the room temperature should be
for storing slides and blocks? We have moved our long term storage out to our
hospitals warehouse and I believe the building is getting much too hot. What
temperature range is everyone using?
Thanks so much
Blake
Hello Histonetters,
Does anyone have experience processing and cutting elephant tissues? Any tips,
tricks, or advice? My lab has had some elephant cases recently and the tissues
are unexpectedly (to us) difficult to cut.
Thanks for any help,
Jennifer Phinney QIHC
Kansas State University
